Class Year
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Priyamvada Rai |
| Class of 1997 Graduated in 2003 | |
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Undergraduate Institution: California Institute of Technology Major: Biology/Chemistry Origin: New Delhi, India | |
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Lab: Stuart Linn and David Wemmer Location: 508 Stanley Hall/ 350T Calvin Lab |
Research
Studies of Fe2+ Localization at and Conformation Anomalies of DNA Consensus Sequences for the Fenton Reaction
I'm studying the conformational variations of DNA sequences RTGR and RGGG and the basis for preferential transition metal localization at these sequences using proton NMR spectroscopy and fluorescence quenching. These sequences are preferentially cleaved by free radicals generated during the ferrous-mediated Fenton reactions, and are found in biologically relevant regions of the genome.
Publications
- Rai P, Cole TD, Wemmer DE, Linn S. Localization of Fe(2+) at an RTGR Sequence Within a DNA Duplex Explains Preferential Cleavage by Fe(2+) and H(2)O(2). J Mol Biol. 2001 Oct 5;312(5):1089-101.
- Henle ES, Han Z, Tang N, Rai P, Luo Y, Linn S. Sequence-specific DNA cleavage by Fe2+-mediated fenton reactions has possible biological implications. J Biol Chem. 1999 Jan 8;274(2):962-71.
- Rai P, Henle E, Linn S. Iron Associates with RTGR-containing sequences in duplex DNA: Implications for Gene Regulation. Free Radic Biol Med. 1998, 25(S1), p.77