Michael Cianfrocco

I am interested in structurally characterizing large macromolecular assemblies through the use of single-particle electron microscopy in the Nogales lab. My research has focused exclusively on determining the cryo-EM structure of human TFIID bound to promoter DNA. While the conformational flexibility of TFIID has made this task a formidable one, we recently had a conceptual & structural breakthrough in our understanding of how structural plasticity relates to TFIID function. This came through the use of ab initio model derivation methods to calculate individual structural states in combination with advanced image processing & refinement strategies. By stepping back and rederiving our 3D models, we gained the objectivity necessary to understand the structural rearrangements necessary for promoter binding by TFIID.

Teaching & science outreach:

"Why not try a scientific approach to science education?" -Carl Wieman, Nobel Prize Physics 1995

Outside of my scientific endeavors in lab, I have become extremely interested in the role that scientists can play in the education of future scientists and the general public. Scientists and educators alike are becoming more aware that the current state of science education in the United States does not reflect the quality of scientific research. While I am just beginning my scientific career, I believe that the future of scientific research will depend upon research scientists taking steps towards the creation of curricula that have the ability to teach ALL students within the classroom.

Cianfrocco, M.A. Hormonal Hassle. Berkeley Science Review Spring 2011

Cianfrocco MA, Kassavetis GA, Grob P, Fang J, Juven-Gershon T, Kadonaga JT, Nogales E. Human TFIID binds to core promoter DNA in a reorganized structural state. Cell 152: 120 - 131. Article.

See also: Highlight by LBNL.